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Thiol-based regulation of redox-active glutamate-cysteine ligase from Arabidopsis thaliana.

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The mean absolute bioavailability observed after IM injections was approximately 14%19% in rats and 45%51% in beagle dogs

the Met protein coding sequence was cloned from rat whole brain cDNA using primers obtained from Integrated DNA the amplified product was gel purified and a band corresponding to 190 kDa band excised and cloned into a PCAGGS-6-Myc destination vector (Gateway)